一、产品名称

Anti-FlagTag (DYKDDDDK) 抗体 (M2)

二、产品描述

FLAG标签，也称为FLAG八肽或FLAG表位，是一种专为融合蛋白设计的标记标签，并拥有专利保护。其显著优势在于能够广泛应用于多种依赖抗体识别的实验中。当没有针对特定蛋白质的专用抗体时，可以通过将FLAG标签添加到该蛋白质上，从而利用抗FLAG序列的抗体进行研究。

FLAG标签的分子量相对较小，仅为1012达尔顿（Da），并且由于其多聚阴离子氨基酸成分，对目标蛋白的功能影响微乎其微。这使得FLAG标签成为理想的研究工具，因为它可以在不干扰蛋白质活性的情况下进行标记及后续研究。此外，FLAG标签可支持高特异性的pull-down实验，这类实验通常具有较低的非特异性背景信号，从而显著提高了检测结果的准确性和可靠性。

三、产品详情

名称：Anti-FlagTag (DYKDDDDK) 抗体 (M2) (RGK08501)
别名：Flagtag, Flagtag (DYKDDDDK-tag), Flag-peptide(DYKDDDDK)
货号：RGK08501
宿主：小鼠
同种型：IgG1, kappa
克隆类型：单克隆
靶标：Flagtag, Flagtag (DYKDDDDK-tag), Flag-peptide (DYKDDDDK)
浓度：156 mg/ml
内毒素水平：请与实验室联系获取信息
纯度：95%（通过SDS-PAGE确定）
纯化方式：通过Protein A/G从细胞培养上清中纯化
克隆号：M2
应用：ChIP, ELISA, EIA, FCM, ICC, IF, IP, WB
状态：液体
保存溶液：0.01 M PBS, pH 7.4
稳定性和存储：建议使用手动解冻的冰箱，避免反复冻融，短期存储在4°C（1-2周），长期存储在-20°C（12个月）或-80°C（长期）。

四、参考文献

1. Sulfation of a FLAG tag mediated by SLC35B2 and TPST2 affects antibody recognition (PMID: 33951064)
2. An internal amino-terminal FLAG-tag octapeptide alters oligomerization of expressed surfactant protein-A (PMID: 32835791)
3. Core-Shell Magnetic Imprinted Polymers for the Recognition of FLAG-Tag peptide (PMID: 36834864)
4. Proteomics-Based Analysis of Protein Complexes in Pluripotent Stem Cells and Cancer Biology (PMID: 27011181)
5. An improved affinity tag based on the FLAG peptide for the detection and purification of recombinant antibody fragments (PMID: 7530459)
6. Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG monoclonal antibodies M1, M2 and M5 (PMID: 9238646)
7. A high-affinity monoclonal antibody against the FLAG tag useful for G-protein-coupled receptor study (PMID: 22465329)
8. Tag-Specific Affinity Purification of Recombinant Proteins by Using Molecularly Imprinted Polymers (PMID: 30786715)
9. Tandem Affinity Purification (TAP) of Interacting Prey Proteins with FLAG- and HA-Tagged Bait Proteins (PMID: 37450137)
10. Hierarchically Imprinted Polymer for Peptide Tag Recognition Based on an Oriented Surface Epitope Approach (PMID: 32990425)
11. High-level expression of anti-FLAG tag antibody in plants (PMID: 38258646)
12. Design and characterization of stimuli-responsive FLAG-tag analogues and the illumination-induced modulation of their interaction with antibody 4E11 (PMID: 16889395)
13. Using Affinity Pulldown Assays to Study Protein-Protein Interactions of Human NEIL1 Glycosylase and the Checkpoint Protein RAD9-RAD1-HUS1 (9-1-1) Complex (PMID: 37574484)
14. Identification of Selenoprotein H Isoforms and Impact of Selenoprotein H Overexpression on Protein But Not mRNA Levels of 2 Other Selenoproteins in 293T Cells (PMID: 34510207)
15. A novel in-capillary assay for dynamically monitoring fast binding between antibody and peptides using CE (PMID: 30367708)
16. Synthesis, purification and bioactivity of recombinant human activin A expressed in the yeast Pichia pastoris (PMID: 19027859)
17. Minimal FLAG sequence useful in the functional epitope tagging of H-Ras (PMID: 12074157)
18. Lithobates catesbeianus (American Bullfrog) oocytes: a novel heterologous expression system for aquaporins (PMID: 29530931)
19. Use of azobenzene amino acids as photo-responsive conformational switches to regulate antibody-antigen interaction (PMID: 19472287)
20. Identification of amino acid residues responsible for the binding to anti-FLAG M2 antibody using a phage display combinatorial peptide library (PMID: 23807489)
21. The human D1 Dopamine receptor: heterologous expression in Saccharomyces cerevisiae and purification of the functional receptor (PMID: 9631523)
22. Tetramerization domain of human butyrylcholinesterase is at the C-terminus (PMID: 9581552)
23. Development of an efficiently cleaved, bioactive, highly pure FLAG-tagged recombinant human Mullerian Inhibiting Substance (PMID: 19755162)
24. Production and purification of human indoleamine 2,3-dioxygenase (HuIDO) protein in a baculovirus expression system and production and characterization of egg yolk antibody against the purified HuIDO (PMID: 16174105)
25. Characterization of capsid-modified adenovirus vectors containing heterologous peptides in the fiber knob, protein IX, or hexon (PMID: 17006548)
26. Topology of the Na(+)/dicarboxylate cotransporter: the N-terminus and hydrophilic loop 4 are located intracellularly (PMID: 11248207)

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